上海芃圣生物科技有限公司

结构式：

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基本信息：

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中文名称：Dibenzocyclooctyne-聚乙二醇-活性酯 (DBCO-PEG-NHS)

别名：DBCO-PEG-NHS

英文名称：DBCO-PEG-NHS，DBCO-PEG-SCM

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：90%以上

应用简介：

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Dibenzocycolctyne (DBCO) PEG NHS derivatives can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneous without the need for toxic Cu catalysts. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Copper-free Click reaction procedures:

Prepare the azide-containing reagents in reaction buffer.

Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.

Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 0C.

Purify conjugates either by dialysis or size-exclusion chromatograph.

物理性质：

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状态：淡黄色到黄色固体，或者半固体，取决于分子量。

溶解性：溶解性：溶于水，氯仿，DMSO等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。NHS可以和NH2。

技术指标：

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纯度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（NHS）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF