上海芃圣生物科技有限公司

结构式：

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基本信息：

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中文名称：Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

别名：FITC-PEG-DBCO

英文名称：FITC-PEG-DBCO，Fluorescein-PEG-DBCO

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

纯度：90%以上

应用简介：

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DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

物理性质：

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状态：淡黄色到黄色固体，或者半固体，取决于分子量。

溶解性：溶于DMSO,DMF等溶剂。

保持条件：-20℃长期保存，避光，干燥。

注意事项：取用一定要干燥，避免频繁的溶解和冻干。

反应性：DBCO于含有N3基团的反应。

技术指标：

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纯度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（FITC）：90%以上

分散系数（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF